CAPN9 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS72122-50, BS72122-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O14815

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is expressed predominantly in stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be associated with gastric cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 79kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of extracts of various cell lines, using CAPN9 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Alternative Name: CAPN9; GC36; nCL-4; calpain-9

Immunogen: Recombinant fusion protein of human CAPN9(NP_006606.1).

Conjugate: Unconjugated

Modification: Unmodified