HPK1-IN-3 is a potent and selective ATP-competitive hematopoietic progenitor kinase 1 (HPK1; MAP4K1) inhibitor with an IC50 of 0.25 nM. HPK1-IN-3 has IL-2 cellular potency with an EC50 of 108 nM in human peripheral blood mononuclear cells (PBMCs).

Product information

Molecular Weight: 490.45

Formula: C23H22F4N6O2

Chemical Name: 4-((2-fluoro-6-(trifluoromethyl)phenyl)amino)-2-((6-methoxy-2-methyl-1, 2, 3, 4-tetrahydroisoquinolin-7-yl)amino)pyrimidine-5-carboxamide

Smiles: COC1=CC2CCN(C)CC=2C=C1NC1=NC(NC2=C(C=CC=C2F)C(F)(F)F)=C(C=N1)C(N)=O

InChiKey: RZLXOEIALAVGKH-UHFFFAOYSA-N

InChi: InChI=1S/C23H22F4N6O2/c1-33-7-6-12-9-18(35-2)17(8-13(12)11-33)30-22-29-10-14(20(28)34)21(32-22)31-19-15(23(25,26)27)4-3-5-16(19)24/h3-5,8-10H,6-7,11H2,1-2H3,(H2,28,34)(H2,29,30,31,32)

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 83.33 mg/mL (169.91 mM; Need ultrasonic).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

HPK1-IN-3 (compound 27; 0.25-4 μM; 24-h) treatment shows a statistically significant elevation of proinflammatory cytokines IL-6 and TNF-α was observed in a concentration-dependent manner in human monocyte-derived dendritic cells.

Products are for research use only. Not for human use.